Last edited by Meztir
Monday, November 30, 2020 | History

2 edition of Cloning of the marine apoptosis inducer, interleukin-1B converting enzyme (ICE), into a yeast expression vector found in the catalog.

Cloning of the marine apoptosis inducer, interleukin-1B converting enzyme (ICE), into a yeast expression vector

Niki Sharan

Cloning of the marine apoptosis inducer, interleukin-1B converting enzyme (ICE), into a yeast expression vector

  • 234 Want to read
  • 15 Currently reading

Published by Laurentian University in Sudbury, Ont .
Written in English


Edition Notes

Statementby Niki Sharan.
The Physical Object
Paginationiv, 41 l. :
Number of Pages41
ID Numbers
Open LibraryOL22162379M

Apoptosis is a highly regulated process and involves a significant number of signaling pathways. Thermo Fisher Scientific offers a wide range of primary antibodies, ELISA kits, multiplexed immunoassays, peptides and recombinant proteins as well as genomic assays targeted to . Apoptosis detection kits We offer a variety of apoptosis detection tools to fit the needs of your system. We have a number of different antibodies and kits geared towards different steps of apoptosis, providing flexibility for apoptosis monitoring.


Share this book
You might also like
Washington Ojers.

Washington Ojers.

Cost-saving techniques in data processing

Cost-saving techniques in data processing

Mechanical properties of degraded PMR-15 resin

Mechanical properties of degraded PMR-15 resin

Imperfect birds

Imperfect birds

drawings and graphic works of Durer

drawings and graphic works of Durer

Regional Research Laboratory Initiative

Regional Research Laboratory Initiative

Patisserie (Le Cordon Bleu)

Patisserie (Le Cordon Bleu)

Two-dimensional numerical simulation of elastic wave propagation for environmental and geotechnical studies

Two-dimensional numerical simulation of elastic wave propagation for environmental and geotechnical studies

Amateur stage management and production

Amateur stage management and production

First follow nature

First follow nature

Annual summary report range studies program

Annual summary report range studies program

Reflections on Russia in the eighteenth century

Reflections on Russia in the eighteenth century

Report to the National Association of Clinical Tutors by the second Wyeth travelling fellow, M. Saunders.

Report to the National Association of Clinical Tutors by the second Wyeth travelling fellow, M. Saunders.

A Practical Handbook for the Treatment of Depression

A Practical Handbook for the Treatment of Depression

Communicating at Work

Communicating at Work

Cloning of the marine apoptosis inducer, interleukin-1B converting enzyme (ICE), into a yeast expression vector by Niki Sharan Download PDF EPUB FB2

Enzyme; this inhibitor is necessary for the virus to suppress the host inflammatory response (7). To pursue the study of this protease, we have cloned a cDNA that encodesaproteolytically activeformofthis converting enzyme.

TheIL converting enzyme was puri-fied from the humanacute monocytic leu-kemiacell line THP-1, and20aminoacidsCited by:   Interleukin-1 beta (IL-1 beta) mediates a wide range of immune and inflammatory responses. The active cytokine is generated by proteolytic cleavage of an inactive precursor.

A complementary DNA encoding a protease that carries out this cleavage has been cloned. Recombinant expression in COS-7 cells enabled the cells to process precursor IL-1 beta to the mature by:   Kayalar C, Ord T, Testa MP, Zhong LT, Bredesen DE.

Cleavage of actin by interleukin 1 beta-converting enzyme to reverse DNase I inhibition. Proc Natl Acad Sci U S A. Mar 5; 93 (5)– [PMC free article] Chen G, Shi L, Litchfield DW, Greenberg AH.

Rescue from granzyme B-induced apoptosis by Wee1 by:   The IL-1 family consists of two pro-inflammatory cytokines viz. IL-1α, IL-1β and a naturally occurring anti-inflammatory agent, the IL-1 receptor antagonist (IL-1Ra or IL-1RN).

The genes encoding these cytokines comprise a cluster spanning Mb on 2qThe two IL-1 isoforms (α and β) are encoded by distinct genes but are structurally related and bind to the same by: Apoptosis is critically dependent on the presence of the ced-3 gene in Caenorhabditis elegans, which encodes a protein homologous to the mammalian interleukin (IL)-1 beta-converting enzyme (ICE).

Interleukin-1 beta converting enzyme is the first member of a new class of cysteine proteases. The most distinguishing feature of this family is a nearly absolute specificity for cleavage at. Apoptosis Inducers. Apoptosis inducers exhibit pro-apoptotic effects through a variety of mechanisms, Cloning of the marine apoptosis inducer DNA cross-linking, inhibition of antiapoptotic proteins and activation of caspases.

These inducers may target a specific cellular process in order to induce antitumor or antineoplastic effects. standing of apoptotic signalling pathways, and the cloning.

Inducers of Apoptosis and Apoptotic Signalling. mammalian interleukin 1 b-converting enzyme. Cell Reports Resource A Small Molecule that Induces Intrinsic Pathway Apoptosis with Unparalleled Speed Rahul Palchaudhuri,1 Michael J.

Lambrecht,1 Rachel C. Botham,1 Kathryn C. Partlow,1 Tjakko J. van Ham,2,3,6 Karson S. Putt,4 Laurie T. Nguyen,4 Seok-Ho Kim,1 Randall T. Peterson,2,3 Timothy M. Fan,5 and Paul J. Hergenrother1,* 1Department of Chemistry, University of Illinois at Urbana.

Activation and Inhibition of Apoptosis Several mechanisms have been identified in mammalian cells for the induction of apoptosis. These mechanisms include factors that lead to perturbation of the mitochondria leading to leakage of cytochrome c or factors that directly activate members of the death receptor family.

Fas is a member of the tumor necrosis factor (TNF) receptor superfamily, a. Interleukin-1β (IL-1β) is a pleiotropic proinflammatory cytokine. Mechanisms leading to its secretion include not only release of newly synthesized pr. Apoptosis, or PCD, is an essential process during the normal development and homeostasis of all multicellular organisms.

Recently, several human and murine ICE or caspase homologies have been cloned (Yuan and Horvitz; ).The ced-3 gene of C. elegans encodes for a cysteine protease that is essential for developmentally-regulated apoptosis. Oligonucleosomal DNA fragmentation, a.

TA cloning is one of the simplest forms of cloning. In this method, vectors containing 5' thymine overhangs are used to accept PCR products in which additional 3' adenosine overhangs have been added on by the nature of TAQ polymerase amplification.

TA cloning has the advantage of ease and speed, since no restriction digestion step is required. Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors June Proceedings of the.

Kuida K, Lippke JA, Ku G, Harding MW, Livingston DJ, Su MS, Flavell RA. Altered cytokine export and apoptosis in mice deficient in interleukin-1 beta converting enzyme. Science. Mar 31; ()– Vaux DL. Toward an understanding of the molecular mechanisms of physiological cell death. Atractyloside is a natural compound that acts both an inhibitor of ANT and an apoptosis inducer.

CSN Apoptosis Activator 2: Apoptosis activator 2 is a potent apoptosis activator which increases procaspase-9 processing and subsequent caspase-3 activation: CSN 9-beta-Arabinosylguanine: Cloning of ced-3 revealed its homology to mammalian interleukin 1-β-converting enzyme (ICE) (, 23).

A distinct ICE-like proteinase responsible for the cleavage of poly(ADP)-ribose polymerase was identified (4), followed by the characterization of a family of ICE-related proteins termed caspases. Caspases are cysteine proteinases. Sgaramella, A.

Bernardi, in Encyclopedia of Genetics, Conclusions. The ability of cloning to yield an exponential multiplication of DNA molecules – in vivo through vector-mediated transformation, as well as in vitro via PCR, is a step adopted in almost all research protocols in experimental genetics (Sambrook et al., ).

DNA cloning has brought about a wealth of knowledge by. Apoptosis is a genetically determined process, by which unwanted or unnecessary cells are eliminated from organisms. Apoptosis is important in many biological contexts and deregulation of apoptosis may cause diseases, either in the case of insufficient apoptosis, which may contribute to excessive cell proliferation and cancer, or excessive apoptosis, which may cause degenerative and.

Cloning and expression of apoptosis inhibitory protein homologs that function to inhibit apoptosis and/or bind tumor necrosis factor receptor-associated factors. Uren AG(1), Pakusch M, Hawkins CJ, Puls KL, Vaux DL. Author information: (1)The Walter and Eliza Hall Institute of Medical Research, Victoria, Australia.

In mammals, IL-1␤ is produced as an inactive precursor that must be cleaved by the IL-1␤ converting enzyme (ICE, also termed caspase-1) to form a biologically active mature peptide (Dinarello.

Interleukin-1β (IL-1β) is the prototypic pro-inflammatory cytokine, whose functions are mediated through interaction with its receptors (IL-1R1 and IL-1R2).

Herein, we cloned the full-length cDNA and genomic DNA of IL-1β and IL-1R2 in the Asian swamp eel (Monopterus albus). The eel IL-1β cDNA encodes a putative polypeptide of amino acids. The protein sequence includes a typical IL. Introduction. Inflammation is one of the first and most important responses of the innate immune system to infection and injury and is known to exacerbate the pathology of major noncommunicable diseases (e.g.

stroke, myocardial infarction, Alzheimer disease, atherosclerosis, diabetes, and cancer) (1, 2).Inflammatory cytokines associated with damaging inflammatory responses are often members of. Pretreatment with poly(I) resulted in complete inhibition of silica- induced apoptosis as measured by cell death ELISA.

Further, we examined the involvement of interleukin-converting enzyme (ICE) in silica-mediated apoptosis using an ICE inhibitor, Z-Val-Ala- Asp-fluoromethyl ketone.

These inducers can be used to induce apoptosis in cell cultures at X dilutions (e.g., Add 1 ml to 1 ml of culture medium). These selected apoptosis inducers have been well characterized that disrupt mitochondrial transmembrane potential, activate caspases, induce phosphatidyl-serine (PS) exposure, DNA fragmentation, and other apoptotic.

Apoptosis inducers are chemical agents that exhibit pro-apoptotic effects through a variety of mechanisms, including DNA cross-linking, inhibition of antiapoptotic proteins and activation of caspases. These inducers may target a specific cellular process in order to induce antitumor or antineoplastic effects, e.g.

Actinomycin D, an antineoplastic antibiotic, inhibits RNA synthesis. The chemical and molecular complexity of the elicitation process renders the precise mechanism of elicitation poorly understood. To date, the mechanism of the action of elicitors is best known in plants and fungal cells (Nützmann et al.,Radman et al., ), but less well known than bacterial current knowledge on the postulated mechanistic underpinnings of elicitation in.

Altered Cytokine ExportandApoptosis in Mice Deficient in Interleukin-1,Converting Enzyme Keisuke Kuida, Judith A. Lippke, George Ku, MatthewW. Harding, David J. Livingston, Michael S.-S. Su,* Richard A. Flavell* Theinterleukin-1P(IL-1 13) converting enzyme(ICE) processestheinactive IL-1 P3 precursor to the proinflammatory cytokine.

The apoptosis process is crucial to various biological processes including embryo development and organism homeostasis. Inducing apoptosis of cancer cells has become a very attractive field for cancer therapy in the recent years.

Tumor necrosis factor–related apoptosis-inducing ligand (TRAIL; also called Apo2L, TNFSF10, CD, or TL2) is a member of tumor necrosis factor family.

interleukin-1b–converting enzyme (FLICE)– pressure overload in the absence of apoptosis (8). Since the cloning of Fas intremen-dous progress has been made in the under-standing of the molecular basis of apoptosis induction by this receptor.

Nevertheless, excellent reviews and books. Chemical induction of apoptosis. Apoptosis inducers act on several apoptosis-related proteins to promote apoptotic cell death. Depending on the agent selected and the concentrations used, apoptotic events can be detected between 8–72 h post-treatment.

However, not all reagents will affect a particular cell line in the same way. Caspases are a large family of evolutionary conserved proteases. The first caspase has been identified as the enzyme necessary for functional maturation of IL-1β.1,2 This molecule, initially named interleukin-1β-converting enzyme (ICE), is the founding member of the caspases:3 the cysteine-containing (in a QACXG-motif) aspartate specific proteases.

Proceed to detect apoptosis using your method of choice. Chemical induction of apoptosis Apoptosis inducers act on several apoptosis-related proteins to promote apoptotic cell death. Depending on the agent selected and the concentrations used, apoptotic events can be detected between 8–72 h post-treatment.

Abstract. Immunohistochemistry is commonly used to show the presence of apoptotic cells in situ. In this protocol, B-cell lymphoma cells are injected into recipient mice and, on tumor formation, the mice are treated with the apoptosis inducer vorinostat (a histone deacetylase inhibitor).

The present invention relates to a cell apoptosis inducer containing chlorine dioxide. The present invention relates to a cell apoptosis inducer kit containing the following two independent components: the first component: a chlorine dioxide precursor solid, or a solution containing a chlorine dioxide precursor; and the second component: an auqeous solution of an acidity pH adjuster; the two.

Expression of Cloned Genes in E. coli Using IPTG-Inducible Promoters (Protocol summary only for purposes of this preview site) Many E. coli expression vectors use regulatory elements derived from the lac operon, which is unsurprising given that the lac operon represents a paradigm for prokaryotic gene regulation (for review, see Reznikoff ).Because the lac promoter itself is relatively.

Apoptosis inducing factor is involved in initiating a caspase-independent pathway of apoptosis (positive intrinsic regulator of apoptosis) by causing DNA fragmentation and chromatin condensation.

Apoptosis inducing factor is a flavoprotein. It also acts as an NADH oxidase. Another AIF function is to regulate the permeability of the mitochondrial membrane upon apoptosis. The DNA of NCI/ADR-RES cells does become fragmented during apoptosis but not extensively enough to form internucleosomal DNA fragmentation.

γ-H2AX was found in NCI/ADR-RES cells undergoing apoptosis (Fig. 1, H andI). Thus, the results obtained with three cell lines and five inducers of apoptosis indicate that the appearance of γ-H2AX is a.

TA cloning is brought about by the terminal transferase activity of certain type of DNA polymerase such as the Taq polymerase. This enzyme adds a single, 3'-A overhang to each end of the PCR product. Ready-to-use high quality apoptosis inducers allow users an easy tool for induction of apoptosis in cultured cells.

These stabilized reagent solutions have a long storage life and are easy to use. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone. Inhibitors of apoptosis are a group of proteins that mainly act on the intrinsic pathway that block programmed cell death, which can frequently lead to cancer or other effects for the cell if mutated or improperly regulated.

Many of these inhibitors act to block caspases, a family of cysteine proteases that play an integral role in apoptosis. Some of these inhibitors include the Bcl-2 family.Niki Sharan has written: 'Cloning of the marine apoptosis inducer, interleukin-1B converting enzyme (ICE), into a yeast expression vector' What is gravity inducer in a mobile phone?Apoptosis Apoptosis is a type of programmed cell death that is critical for numerous normal physiological processes.

Historically, apoptosis has been defined by its morphological features, most of which were described in the s by John Kerr 2. Apoptotic morphology includes cell shrinkage, membrane blebbing, chromosome condensation.